PLATELET-derived growth factor B (PDGFB) improves follicular development and ovarian angiogenesis in rar model of polycystic Ovary Syndrome

PARBORELL FERNANDA; IRUSTA GRISELDA; ABRAMOVICH DALHIA; TESONE, MARTA; PASCUALI NATALIA; SCOTTI LEOPOLDINA; DI PIETRO MARIANA

Congreso; 17th world congress of human reproduction; 2017

Context: Patients with Polycystic Ovary Syndrome(PCOS) have alterations in ovarian angiogenesis. The most studied of thesealterations is the increase in vascular endothelial growth factor (VEGF)production by ovarian cells. Platelet-derived growth factor B and D (PDGFB andD) are decreased in follicular fluid of PCOS patients and in the ovaries of arat model of PCOS. Therefore, we hypothesized that restoration of ovarian PDGFBlevels can improve ovarian angiogenesis and follicular development in a ratmodel of PCOS. Objective: We aimed to analyze the effects of localadministration of PDGFB on ovarian angiogenesis, follicular development andovulation in a DHEA-induced PCOS rat model. Methods: Prepubertal female SD ratswere injected sc with DHEA (6 mg per 100 g body weight) daily for 15 d toinduce PCOS. Control animals were injected with 0.2 ml of sesame oil. For PDGFBadministration, DHEA-treated rats were injected under the bursa of both ovarieswith 25 ng of PDGFB on day 15 of DHEA treatment. Another group received saline.On day 16 rats were sacrificed and the ovaries collected for further studies.Main Outcome measures: follicular development, AMH, endothelial andperiendothelial cell areas, angiogenic factor levels. Results: Ovarian PDGFBadministration to PCOS rats partially restored follicular development,decreased the percentage of cysts and AMH levels and increased the percentageof corpora lutea. In addition, PDGFB improved ovarian angiogenesis by reversingthe increase in periendothelial cell area and restoring VEGF levels.Conclusion: This study demonstrates a beneficial effect of local PDGFBadministration on luteal development and ovarian angiogenesis in a rat model ofPCOS. These results contribute to the knowledge of the ovarian mechanisms thatlead to altered ovarian function in PCOS and provide new data for potential therapeuticstrategies.