Resveratrol reduces doxorubicin-induced ovarian damage in mice ovary

ABRAMOVICH D; N PASCUALI; L SCOTTI; OUBIÑA G,; F. PARBORELL; DI PIETRO M

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias, Buenos Aires; 2017

Sociedades Argentinas de Biociencias

Premature Ovarian Failure (POF) is characterized by the disappearance of ovarian follicles in young women, which may be caused by chemotherapy. POF treatments such as hormone therapies are not completely effective. We evaluated whether Resveratrol (Rs) can prevent gonadotoxicity caused by doxorubicin (Dx) in a mouse model. F1 mice (BalbC x C57 / BL6) (8 weeks) were given either an single intraperitoneal injection of Dx (10mg/kg) or saline solution.Control and Dx mice underwent sham surgery and received an intrabursal injection of saline solution, while Dx+Rs groups received 1μM or 10μM of Rs intrabursa, 1h prior to Dx administration. Animals were sacrificed 2 weeks after surgery (n=5-8/ group) and ovaries were removed, one for Western Blot (WB) and the other for histological analysis. Paraffin-embedded ovarian slides were stained in H&E for morphological study or immunostained for DDX4 (germ-line cell specific marker) and AMH (ovarian reserve marker). Results were evaluated by ANOVA followed by Dunnet?s test. Dx reduced the % of primary follicles compared to control (p