IN VITRO STUDY OF TWO NATURAL COMPUNDS FOR ENDOMETRIOSIS TREATMENT: ELLAGIC AND RETINOIC ACID

RICCI, ANALÍA GABRIELA; FERELLA, LUCIANA; BARAÑAO, ROSA INÉS; BILOTAS, MARIELA ANDREA; MCCORMACK, BÁRBARA; BARAÑAO, ROSA INÉS; BILOTAS, MARIELA ANDREA; MCCORMACK, BÁRBARA; SINGLA, JOSÉ JAVIER; MADANES, DANIELA; SINGLA, JOSÉ JAVIER; MADANES, DANIELA; RICCI, ANALÍA GABRIELA; FERELLA, LUCIANA

Congreso; 33th Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE); 2017

European Society of Human Reproduction and Embryology (ESHRE)

Study question: In this work we propose to assess the in vitro effect of two natural compounds: ellagic acid (EA) and retinoic acid (RA), on human endometrial cell adhesion.Summary answer: EA and RA significantly reduced cell adhesion of human endometrial stromal cell line (T-HESC) and endometrial epithelial carcinoma cell line (ECC-1).What is known already: EA is a natural poliphenol, which has been widely demonstrated to have properties as antioxidant and anti-tumoral in-vitro and in-vivo. RA is the active metabolite of vitamin A (retinol). It is known that retinoids have many biological effects, including a variety of immunomodulatory and anti-inflammatory activities, and have already shown satisfactory results in cancer research. In our previous work was demonstrated that 10µM RA significantly inhibited T-HESC and ECC-1 proliferation, while 100µM EA inhibited ECC-1 proliferation and no interfere with T-HESC viability. In the same way, we observed a significant tendency towards T-HESC cycle arrest after RA and EA treatment.Study design, size, and duration: Both cell lines, T-HESCs and ECC-1, were incubated with EA or RA for 24h, and subsequent evaluated for plastic adhesion.Participants/materials, setting, and methods: Cell adhesion was evaluated by plastic adhesion assay in ECC-1 and T-HESC cultures. Briefly, cells were treated with 50-100 µM EA and 5-10 µM RA. After 24h cells were trypsinized and allowed to recover for 40min in fresh serum-free medium. Then were sowed and 60min later adhered cells were washed, trypsinized and counted.Main results and the role of chance: We observed that 24h treatment reduced plastic adhesion of both cell lines under study. ECC-1 treated with 50 and 100 µM EA significantly reduced their plastic adhesion ability (p