Effects of estrogen membrane receptor GPR30 agonist on hippocampal astrogliosis and microgliosis of Spontaneously Hypertensive Rats

DE NICOLA, ALEJANDRO F.; LABOMBARDA, FLORENCIA; PIETRANERA, LUCIANA; ROIG, PAULINA; LIMA, ANALÍA; CORREA, JULIETA

Buenos Aires

Encuentro; 1st South American Glia Meeting; 2017

Hippocampal neuropathology is a recognized feature of the spontaneously hypertensive rat (SHR). Previous studies have found that SHR present abnormalities in the hippocampus consisting of decreased cell proliferation in the dentate gyrus (DG), astroglial and microglial reactivity and decreased neuronal density in the hilus of the DG. These abnormalities are reversed by exogenous administration of estradiol, an active neuroprotective and hypotensive factor. We recently demonstrate that both types of estradiol classical receptors (ERand ER)are involved in the neuroprotective action of estradiol. GPR30 is a membrane estradiol receptor which is expressed in hippocampus and it is known to be involved in neuroprotective actions. To elucidate if this receptor is involved in the protective effects in SHR we used the GPR30 agonist G1 given s.c. during 2 weeks at 150 µg/kg/day to 4 month old male SHR. We measured the expression of GFAP and IBA1 in the CA1 and hilus of the hippocampus by immunocytochemistry. We found that hypertensive animals have increased expression of GFAP+ astrocytes in both regions of the hippocampus (p