IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Novel angiogenic role of an endogenous lectin involved in adipogenesis is mediated by specific glycoepitopes
Autor/es:
SUNDBLAD, VICTORIA; CAGNONI, ALEJANDRO J.; MARIÑO, KARINA V.; JUAN MANUEL PÉREZ SÁEZ; RABINOVICH, GABRIEL A.; CROCI, DIEGO O.; MALLER, SEBASTIÁN M.
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
The continuous advance of metabolic syndrome, and its comorbidities (type 2 diabetes, cardiovascular disease) brought adipose tissue research back to the spotlight. In response to caloric excess, adipose tissue remodeling leads to chronic inflammation, with persistent stress and/or impaired resolution process. In turn, these phenomena result in functional maladaptation and adipocyte hypertrophy, increased angiogenesis, immune cell infiltration, and extracellular matrix overproduction. The cellular and molecular events that are involved in the systemic chronic inflammatory state associated with obesity are not completely clear, and a deeper insight into molecular mediators of these processes may reveal clinically relevant pathways that control AT inflammation.Galectin-12 (Gal-12) is a tandem-repeat member of the galectin family, preferentially expressed in lipid droplets of adipocytes, and its ability to supress lipolysis has attracted considerable interest. We have cloned and expressed recombinant murine Gal-12 (mGal-12) in Escherichia coli, and biochemically characterized it as a lectin with affinity for Lewis X structures. In this study, we demonstrate for the first time the pro-angiogenic role of this lectin in transwell migration assays with HUVEC cells, which can be eliminated by competition withby specific glycan ligands. Finally, mGal12 expression is also stimulated under low oxygen culture conditions, as its expression in 3T3L1 adipocytes is modulated by hypoxia. Together, these data indicate that mGal12 not only regulates adipogenesis but also angiogenesis in adipose tissue, providing a new potential target for the development of specific therapies for obesity-related disorders.