In-Vitro Effects of Benzophenone 2 and 3 Immature GnRH Neurons

LUX-LANTOS V; FERNÁNDEZ M; AROCENA P; FERNÁNDEZ M; AROCENA P; LIBERTUN CARLOS;; LIBERTUN CARLOS;; LUX-LANTOS V

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. 13 a 17 de noviembre 2017.; 2017

In-Vitro Effects of Benzophenone 2 and 3 Immature GnRH NeuronsArocena P, Libertun C, Lux-Lantos V, Fernandez MReunion Conjunta de Sociedades de Biociencias. Buenos Aires. 13 a 17 de noviembre 2017.Medicina vol 77 Suplemento 1. 2017. Pag 390. Abstract 69.UV filters, such as benzophenones (BPs), are used as additivesin plastics, textiles and personal care products to prevent damagecaused by UV radiation. Here we investigated the in-vitro effectsof two commonly used benzophenones, BP2 and BP3, on cell proliferationand on basal and kisspeptin-stimulated GnRH release inimmature GnRH neurons, GN11 cells.Cell proliferation was studied using the MTS assay (Promega)in response to 24 hour treatment with BP2, BP3 (10-9 and 10-7 MSigma), the estrogen antagonist ICI 182780 (ICI, 10-6 M, Sigma)and kisspeptin (Kiss1, 10-9 M, Phoenix Pharmaceuticals). Resultswere recorded as Abs490/Abs490 (Control). Basal and Kiss1 (10-9M, 1h)-stimulated GnRH release was studied after 24 h exposure toBP2 or BP3 (10-9 and 10-7 M). GnRH release was analyzed by RIA.Results were expressed as Mean±SEM, and analyzed by ANOVA.BP2 and BP3 increased cell proliferation compared to Controls (C)[C: 1.0±0.1, BP2-7: 1.3±0.2, BP2-9: 1.4±0.1, BP3-7: 1.5±0.1, BP3-9:1.5±0.1; BP2, BP3 different from C, p