IMPACT OF IMT504 ON THE IMMUNE SYSTEM OF FEMALE NON-OBESE DIABETIC (NOD/Ltj) MICE

CARLOS LIBERTUN; MARIA SILVIA BIANCHI; VERONICA MARTINEZ ALLO; VICTORIA LUX-LANTOS; STEFANIA BIANCHI; MARTA TOSCANO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC-SAIB-SAI-SAA-SAB-SAB-SAFE-SAFIS-SAH-SAP

Immunomodulatory oligonucleotide IMT504 (IMT) improved glycemia, beta cell function, and reduced leukocyte islet infiltration in spontaneous autoimmune diabetic mice (female NOD/Ltj). We analyzed the effects of IMT on immune parameters. Female diabetic NOD/LtJ mice (two consecutive glycemia levels (Gly) ≥ 250 mg/dl)were treated daily with IMT for five days (20mg/kg/day) or saline as control (DC). The day following the last injection, fasted Gly was measured and mice were sacrificed. Pancreatic leukocytes and splenocytes were obtained, immune cell populations (by flow cytometry) and cytokines expression (by q PCR) were analyzed respectively. DC mice showed 11% (2/19) spontaneous reversion of the diabetic condition whereas IMT treatment improved Gly in 72% of mice (13/18) (χ2: DC vs IMT20: p