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Study question: In this work we propose to assess the in vitro effect of twonatural compounds: ellagic acid (EA) and retinoic acid (RA), on human endometrialcell adhesion.Summary answer: EA and RA significantly reduced cell adhesion of humanendometrial stromal cell line (T-HESC) and endometrial epithelial carcinomacell line (ECC-1).What is known already: EA is a natural poliphenol, which has been widelydemonstrated to have properties as antioxidant and anti-tumoral in-vitro andin-vivo. RA is the active metabolite of vitamin A (retinol). It is known that retinoidshave many biological effects, including a variety of immunomodulatoryand anti-inflammatory activities, and have already shown satisfactory results incancer research. In our previous work was demonstrated that 10 μM RA significantlyinhibited T-HESC and ECC-1 proliferation, while 100 μM EA inhibitedECC-1 proliferation and no interfere with T-HESC viability. In the same way,we observed a significant tendency towards T-HESC cycle arrest after RA andEA treatment.Study design, size, duration: Both cell lines, T-HESCs and ECC-1, wereincubated with EA or RA for 24 h, and subsequent evaluated for plasticadhesion.Participants/materials, setting, methods: Cell adhesion was evaluatedby plastic adhesion assay in ECC-1 and T-HESC cultures. Briefly, cells weretreated with 50-100 μM EA and 5-10 μM RA. After 24 h cells were trypsinizedand allowed to recover for 40 min in fresh serum-free medium. Thenwere sowed and 60 min later adhered cells were washed, trypsinized andcounted.Main results and the role of chance: We observed that 24 h treatmentreduced plastic adhesion of both cell lines under study. ECC-1 treated with 50and 100 μM EA significantly reduced their plastic adhesion ability (p < 0.01 andp