Active biomolecules present in Scutellaria baicalensis and Rosmarinus officinalis induce cell cycle arrest in in-vitro model of endometriosis.

FERELLA L; MC CORMACK B; OLIVARES C; BASTON JI; SINGLA JJ; MERESMAN G; BILOTAS M; GONZALEZ A

Genova

Congreso; 33rd Annual Meeting of the Eurpean Society of Human Reproduction and Embryology (ESHRE); 2017

ESHRE

Study question:The objective was to evaluate the effect of Wogonin (WG), Carnosic Acid (CA) and Rosmarinic Acid (RA) on human endometrial stromal cell line (t-HESC) growth.Summary answer:Results suggest that inhibition of t-HESC proliferation by CA and WG might be associated to cell cycle arrest induction in G2/M phase.What is known already:Current medical therapies available for endometriosis result inefficient due to their long term side effects and high levels of recurrence. Natural compounds are being investigated because of their beneficial properties. WG, a flavonoid isolated from Scutellaria baicalensis root, is the most active constituent of Chinese Herbal Medicine. CA and RA are two of the main antioxidant compounds found in Rosmarinus officinallis leaves. Anti-tumoral effects of these polyphenols have already been reported in several types of tumor cell lines. Previous studies developed in our laboratory demonstrated that these compounds inhibit endometrial stromal cell proliferation in both in-vitro and in-vivo endometriosis model.Study design, size, duration:T-HESC cell line was incubated with WG, CA or RA for 24 hours or 48 hours for apoptosis assay or cell cycle analysis respectively. After incubation, cells were harvested and fixed, and TUNEL technique or propidium iodine staining was performed. Apoptosis and cell cycle analysis were measured by flow cytometry. Participants/materials, setting, methods:After 24-h incubation with CA (5 and 7.5 µg/ml), RA (50 and 100 µg/ml) and WG (40 and 80 µM), cells were fixed to assess the percentage of apoptosis by the TUNEL technique. After 48-h incubation with CA (2.5 and 5 µg/ml), RA (50 and 100 µg/ml) and WG (40 and 80 µM), cells were fixed and DNA was stained with propidium iodine. Subsequently, apoptosis and cell cycle were measured in t-HESC by flow cytometry. Main results and the role of chance:Pro-apoptotic effect of WG, CA and RA has not been evidenced by TUNEL technique in t-HESC cell line after 24-h incubation with each compound. WG 40 and 80 µM significantly decreased the percentage of cells in the G0/G1 phase and complementarily increased the percentage of cells in G2/M phase (p