Endometriosis alters the expression of steroidogenic proteins in the ovary and the release of steroid hormones in a rat model

MADANES D; SCALISE G; BARAÑAO R.I.; OLIVARES C.; BIZZOZZERO M; BENGOECHEA T; PARBORELL F; MC CORMACK B.; BILOTAS M; SCHEFFER F; RICCI A.

C.A.B.A.

Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

SAB-SAIC-SAIB-SAI-SAA-SAH-SAP-SAB-SAFE-SAFIS

Objetive: To study the expression of steroidogenic proteins, the release of estradiol and progesterone, the ovulation and luteal size in the ovary of rats with experimentally induced endometriosisMaterials and Methods: Endometriosis was surgically induced in Sprague Dawley rats by autotransplantation of uterine horn pieces to the bowel mesothelium. Sham animals were used as controls. Rats were sacrificed one month after surgery at different stages of menstrual cycle (proestrus, estrous and diestrus). Luteal size was measured in ovary sections stained with hematoxylin-eosin. Aromatase P450, P450scc, 3β-HSD and StAR expression was assessed by western blot in isolated follicles and corpora lutea (CL). Estradiol and progesterone serum levels were evaluated by RIA. Ovulated oocytes were counted in the ampulla in the morning of estrous. Results: StAR expression decreased in follicles from rats with endometriosis in all estrous cycle stages (p