Bioactive sphingolipid Ceramide-1-phosphate (C1P) protects against age-related ovarian dysfuntion/decline in female mice

LEOPODINA SCOTTI; N.PASCUALI, M. DI PIETRO, G. OUBIÑA, D. COHEN, D. ABRAMOVICH AND F. PARBORELL; LEOPODINA SCOTTI; N.PASCUALI, M. DI PIETRO, G. OUBIÑA, D. COHEN, D. ABRAMOVICH AND F. PARBORELL

Congreso; 17 th World Congress of the Academy of Human Reproduction; 2017

Context: As many women postpone childbearing over 38 years, a large portion of aged female becomes infertile. Ovarian aging is dominated by the progressive loss of primordial follicles and a decline in oocyte quality. Activation of ovarian angiogenesis has emerged as a new strategy to halt age-related decline of ovarian response. Bioactive sphingolipids such as C1P, a proangiogenic and antiapoptotic factor, are key regulators of cell homeostasis. Objective: To study whether C1P can improve the ovarian response and angiogenesis in aged female mice.Methods: Aged female mice (26-31 weeks) received C1P (10µl/ovary; 50µM) under the bursa of one ovary and vehicle on the other, and were sacrificed 48h later. Young mice (6-9 weeks) were used as control. Ovaries were isolated.Main Outcome Measure(s): Histological and immunohistochemical analysis (VW and α-SMA; endothelial and periendothelial cell markers, respectively), radioimmunoassay (E2 and P4 concentrations) and western blot (pFoxo3a/Foxo3a and AMH; ovarian reserve markers) were performed. Results: The % of primary (PF), preantral (PrF) and antral follicles (AF) in aged mice were lower than in young mice (p