LEPTIN ACTION ON TROPHOBLASTIC CELLS SURVIVAL UNDER HYPOXIA CONDITION

BALESTRINI, PAULA; JAIME, MARIANA; VARONE, CECILIA LAURA; TORO, AYELÉN; MASKIN, BERNARDO; PÉREZ-PÉREZ, ANTONIO; SÁNCHEZ-MARGALET

Mar del Plata

Congreso; Sociedad Argentina de Investigaciones Clínicas; 2017

Sociedad Argentina de Investigación Clínica (SAIC)

Leptin is produced by placenta and has many roles as an autocrine hormone. We havepreviously demonstrated that leptin promotes proliferation and survival of trophoblastic cells. Inthe present work we aimed to study the mechanisms that mediate the effect of leptin in placentalapoptosis induced by cobalt chloride (CoCl 2 ), a hypoxia mimicking agent. We determined whetherleptin modulates p53 expression and activity of placental cells, and also studied the regulation ofHIF-1α. Swan-71 cells, cultured under standard conditions, as well as, human placenta explantswere used. Placental cells and explants were treated with CoCl 2 (50, 100 and 250 µM) to induceapoptosis and with or without 100 ng/ml of recombinant leptin. Western blot and transfectionassays with a p53 reporter constructs vectors were carried out. All procedures were approved byethical review committee at the A Posadas National Hospital. In order to prove CoCl 2 effect, westudied HIF-1α expression and we confirmed that the treatment with 100 µM of CoCl 2 induced itsexpression (p