IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IDENTIFICATION OF SOLUBLE COMPONENTS IN HUMAN ADIPOSE TISSUE EXPLANTS FROM RENAL CELL CARCINOMA
Autor/es:
ROMEO LEONARDO R; LÓPEZ FONTANA RODRIGO; ZYLA LEILA; PISTONE CREYDT VIRGINIA; LÓPEZ LAUD JD; LÓPEZ FONTANA GASTÓN; SASSO CORINA VERÓNICA; CARÓN RUBÉN; CAMPOVERDE ARBOCCO FIORELLA; GIORLANDO NOELIA; SANTIANO FLAVIA; LÓPEZ FONTANA CONSTANZA
Lugar:
Mendoza
Reunión:
Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016
Resumen:
We have recently demonstrated that conditioned media (CMs) from human adipose tissue explants from renal cell carcinoma near the tumor (hRATnT) regulate adhesion and migration of tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells, contrary to CMs from human adipose tissue explants from renal cell carcinoma farther away from the tumor (hRATfT). Now, we have begun to identify soluble components present: 1) in hRATnT- and hRATfT-CMs by WB; 2) in tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells incubated with different CMs. We employed the CMs after 24 h of incubation of hRATnT and hRATfT. We evaluated the expression of proteoglycan versican, metalloproteinase ADAMTS1, leptin and adiponectin in the CMs. We incubated 786-O, ACHN and NK-2 cells with theCMs for 24 h, lisated the cells and evaluated the expression of AdipoR1, AdipoR2, ObR and leptin. We observed a higher expression of versican, leptin and adiponectin, as well as a lower expression of ADAMTS1 in hRATnT-CMs compared to the expression in hRATfT-CMs (p