Differential gene expression associated to migratory, secretory and morphogenetic processes, and VEFG-mediated signaling in osteosarcoma cell lines that differ in their metastatic ability

AMOROS MA ; KLEINERMAN ES; BOLONTRADE MF; GUTIERREZ LM; MAZZOLINI G; CORREA DOMINGUEZ A; BAYO J; GARCIA MG

Mar del Plata

Congreso; LXI Reunión Anual SAIC SAI SAFE; 2016

SAIC SAI SAFE

Despite combination of chemo- and radiotherapy, survival rate for osteosarcoma (OS) remains 60-70%. OS presents major therapeutic challenges at lung metastasis stage with a survival rate of 15-30% at diagnosis time. We used a metastatic OS model that includes a subline (LM7) with enhanced lung metastatic ability. Proteomic analysis identified differentially expressed proteins both in the secretory and cellular compartments of the metastatic and parental (SAOS2) cell lines. We identified an increased gene expression associated to VEGF signaling, morphogenesis and tissue remodeling and migratory ability in the metastatic subline. A functional profile evaluation revealed no significant differences between both cell types secretome´s ability to induce angiogenesis in vitro, suggesting that a differential role for VEGF could be associated to non-angiogenic related mechanisms. LM7 showed increased in vitro migratory behavior; moreover, mesenchymal stem cells (MSC) displayed enhanced migration towards the parental secretome (1200 ±98 vs 820±158 cells/field, SAOS2 vs LM7 respectively, p