Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression in TM4 Sertoli cells

MATZKIN ME; MAYERHOFER A; ROSSI SP; REY V; FRUNGIERI MB; WINDSCHUETTL S; CALANDRA RS

St Malo

Workshop; 19th European Testis Workshop; 2016

Among Sertoli cell functions that are important to germ cell development is the provision of adequate levels of energy substrates such as lactate. The lactate production is result of the conversion of pyruvate to lactate catalyzed by the enzyme lactate dehydrogenase (LDH).Cyclooxygensase 2 (COX2), the inducible isoenzyme of prostaglandin biosynthesis, isexpressed in different testicular cell populations including Sertoli cells (Frungieri et al., Reproduction 49: R169, 2015). Sertoli cells produce prostaglandin D2 (PGD2) and express DP receptors (Samy et al., Endocrinology 141: 710, 2000; Moniot el al., Development 136:1813, 2009; Matzkin et al. Gen. Comp. Endocrinol. 179: 254, 2012).The role of PGD2 in the response of Sertoli cells to oxidative stress has not been explored so far.The present study therefore attempted to investigate the participation of the PGD2 system in the modulation of the oxidant-antioxidant status in Sertoli cells. Furthermore, the involvement of PGD2 -dependent reactive oxygen species (ROS) generation in the physiological regulation of Ldh a gene expression in these somatic cells has also been investigated.