Exploring the role of epinephrine in human testicular peritubular cells

REY V; FRUNGIERI MB; ROSSI SP; MAYERHOFER A

Mar del Plata

Congreso; LXI Reunion de la Sociedad Argentina de Investigacion Clinica, SAIC; 2016

Sociedad Argentina de Investigación Clínica, SAIC

Catecholamines reach the testes via its sympathetic innervation and theblood stream. They mediate stress-responses via receptors and may be areinvolved in the regulation of testicular function. We determined by RT-PCR thatHuman Testicular Peritubular Cells (HTPCs) isolatedfrom infertile patients express α1-, β1- and β2- adrenergic receptors. The aimof this study was to investigate if HTPCs are possible targets ofcatecholamines and to test their influence on cell viability, smooth musclemarkers, pro-inflammatory factors and CXCL12 expression.Epinephrine (Epi; 10nM, 100nM, 1μM and 10 μM) has a trophic effect andsignificantly increases metabolic activity (determined by ATP assay) on HTPCs,after 24 h of incubation.Epi and 10μM salbutamol (S; agonist β2) and phenylephrine (P; agonistα1) decreased calponin and smooth muscle actin (SMA) mRNA expression after 24 hincubation (qPCR: calponin/GAPDH: B:1.00±0.09; Epi:0.63±0.08 ;S:0.68±0.12 ; P:0.63±0.06 ; SMA/GAPDH: B:1.00±0.08;Epi:0.39±0.10 ; S:0.51±0.02 ; P:0.52±0.02 ;X±SEM; * P