IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Reactive oxygen species (ROS) generation triggered by PGD2 in Sertoli cells
Autor/es:
FRUNGIERI MB
Lugar:
Munich
Reunión:
Seminario; Seminarios en el Biomedical Center (BMC), Cell Biology, Anatomy III, LMU, Munich, Alemania; 2016
Institución organizadora:
Biomedical Center (BMC), Cell Biology, Anatomy III, LMU, Munich, Alemania
Resumen:
Reactive oxygen species (ROS) regulate testicular function in health and disease.We previously described a prostaglandin D2 (PGD2) system in Sertoli cells. Now, we found that PGD2 increases ROS and hydrogen peroxide (H2O2) generation in murine TM4 Sertoli cells, and also induces antioxidant enzymes expression suggesting that defense systems are triggered as an adaptive stress mechanism that guarantees cellsurvival. ROS and specially H2O2 may act as second messengers regulating signal transduction pathwaysand gene expression. We describe a stimulatory effect of PGD2 on lactate dehydrogenase (LDH) expression via DP1/DP2 receptors, which is prevented by the antioxidant N-acetyl-L-cysteine and the PI3K/Akt pathway inhibitor LY 294002. PGD2 also enhances Akt and CREB/ATF-1 phosphorylation.Our results provide evidence for a role of PGD2 in the regulation of the oxidant/antioxidant status in Sertoli cells and, more importantly, in the modulation of LDH expression which takes place through ROS generation and the Akt-CREB/ATF-1 pathway