Culturing cells in microchannels for monoclonal antibodies production.

PAYÉS, CRISTIAN; LASORSA, CARLOS; SIERRA-RODERO, MARINA; LERNER, BETIANA; PEÑAHERRERA, ANA; KARP, PAOLA; ATTALLAH, CAROLINA; GOLMAR, FEDERICO; PEREZ, MAXIMILIANO; BOURGUIGNON, NATALIA; ROSERO, GUSTAVO; GRANELL, PABLO; HELGUERA, GUSTAVO

La Plata

Congreso; XIV Reunión sobre Recientes Avances en Física de Fluidos y sus Aplicaciones.; 2016

División de Fluidos y Plasmas - Asociación Física Argentina

Monoclonal antibodies production for therapeutic use is a growing industrial area. Production in mixed reactors faces challenges related with the quality of product, glycosylation control and reproducibility. One approach to improve the control is to reduce the scale of the production platform through microdevices [1]. Anti-FN-a2b is a chimeric molecule as it has scFv region from a murine model and the Fc region from a human origin[2]. These recombinant antibodies show neutralizing ability of antiviral and antiproliferative activity of IGN-a2b, in vitro. Some autoinmmune diseases like systemic erythematosus lupus progresses with high levels of IFN-a, affecting the life quality of patients. In this study, two types of cells that produce the antibody, HEK-293 and CHO-K1 were cultured in poly-dimetil silaxane (PDMS) microdevices bonded to glass. Microchannels were covered with poly-lysine to attach cells to microchannels and different geometries have been tested, narrow and wide microchannels. Cell covered area was measured and it was determine that wide channels presented higher cell growth[3] . Furthermore, cell distribution was evaluated through Vesicle stomatitis virus (VSV) infection and subsequent Green Fluorescent Protein (GFP) expression.