In Vitro Effects of Bisphenol a in Immature GnRH Neurons

AROCENA PAULA; FERNANDEZ MARINA; LIBERTUN CARLOS; LUX-LANTOS VICTORIA

Orlando

Congreso; ENDO2017; 2017

The Endocrine Society

Bisphenol A, (BPA), a component of polycarbonate plastics, epoxy resins and polystyrene found in many common products, is an endocrine disruptor that alters several functions in different species, including rats, mice and humans. Previously we described the effects of neonatal exposure to BPA on the hypothalamic-pituitary-gonadal axis of female rats (1;2). In this study we analyzed the in vitro effects of BPA in immature GnRH neurons, GN11 cells, developed by Dr. Susan Wray (NIH), and provided by Dr. Pamela Mellon (UCSD), USA.We studied cell proliferation using a Non-Radioactive Cell Proliferation Assay, MTS (Promega, WI, USA) in response to BPA (1x10-9 and 1x10-7M, Sigma Aldrich, MO, USA), estradiol (E2, 1x10-9and 1x10-7M, Sigma), the estrogen antagonist ICI 182780 (ICI, 1x10-6M, Sigma) and kisspeptin-10 (Kiss, 1x10-9M, Phoenix Pharmaceuticals Inc., CA, USA). Results were recorded as Abs490/Abs490(Control), presented as Mean±SE and analyzed by ANOVA with a Fisher posttest (Statistica, StatSoft, OK, USA).Twenty four h treatment with BPA and E2 increased cell proliferation relative to control (Control: 1±0.1, BPA 1x10-7M: 1.4±0.1, BPA 1x10-9M: 1.6±0.3, E2 1x10-7: 1.7±0.2, E2 1x10-9:1.6±0.2; BPA, E2 different from Control, n=9, p