Antihistamines and corticoids co-treatment rationale. Potentiation of glucocorticoids anti-inflammatory effects

SOTO A; SHAYO C; MONCZOR F; GRANJA GALEANO G; GOLDMAN A; ZAPPIA C; FERNANDEZ N; FITZSIMONS CP

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXIV Reunión Anual Sociedad Argentina de Inmunología (SAI) y XLVIII Reunion Anual Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAIC

Antihistamines and glucocorticoids are used to treat many inflammatory conditions, such as allergic rhinitis and asthma, and in many cases they are co-administered. However, this association has no clear rationale and has arisen from clinical practice. We have previously reported the potentiating effects of histamine H1 receptor signaling on glucocorticoid receptor (GR) activity in vitro. Here we evaluated the effect of the H1 antihistamine azelastine (Aze) on GR transcriptional activity induced by Dexamethasone (Dex). HEK293T cells were co-transfected with plasmids coding for GR, H1R and a GR-responsive luciferase reporter plasmids to study transactivation (TAT3-Luc) or transrepression (IL6-Luc) of responsive genes. In both cases, preincubation with 10 μM Aze induced a three-fold increment in the response elicited by 0.1 nM Dex (p