IN-VITRO EFFECTS OF BISPHENOL A IN IMMATURE GNRH NEURONS

AROCENA P; FERNANDEZ MO; LIBERTUN C; LUX LANTOS V

Buenos Aires

Jornada; Jornadas SAB 2016; 2016

Sociedad Argentina de Biología

IN-VITRO EFFECTS OF BISPHENOL A IN IMMATURE GNRH NEURONSFernández M, Arocena P, Lux-Lantos V and Libertun C. Laboratorio de Neuroendocrinología (IByME-CONICET), Facultad de Medicina (UBA). E-mail: mfernandez@dna.uba.ar.Bisphenol A, (BPA), a component of polycarbonate plastics, epoxy resins and polystyrene found in many common products, is an endocrine disruptor that alters several functions in different species, including rats, mice and humans. Previously we described the effects of BPA on the hypothalamic-pituitary-gonadal axis of female rats. In this study we analyzed the in vitro effects of BPA in immature GnRH neurons, GN11 cells, developed by Susan Wray, USA. We studied cell proliferation using a Non-Radioactive Cell Proliferation Assay, MTS (Promega, WI, USA) in response to BPA (1x10-9 and 1x10-7M) and estradiol (E2, 1x10-9and 1x10-7M). Results were recorded as Abs490/Abs490(Control), presented as Mean±SE and analyzed by ANOVA with a Fisher posttest (Statistica, StatSoft, OK, USA). Twenty four h treatment with BPA and E2 increased cell proliferation relative to control (Control: 1±0.2, BPA 1x10-7M: 1.5±0.2, BPA 1x10-9M: 1.9±0.4, E2 1x10-7: 1.9±0.4, E2 1x10-9:1.7±0.3, n=5, p