EFFECTS OF GABAB ANALOGS ON BRAIN KISS1 EXPRESSION IN ADULT MALE MICE

BOURGUIGNON NS; LUX LANTOS V; BIZZOZZERO MARIANNE; DI GIORGIO NP; LIBERTUN, C; TABARES FLORENCIA

Buenos Aires

Jornada; Jornadas SAB 2016; 2016

Sociedad Argentina de Biología

EFFECTS OF GABAB ANALOGS ON BRAIN KISS1 EXPRESSION IN ADULT MALE MICEDi Giorgio N, Bizzozzero M, Bourguignon N, Tabares F, Libertun C and Lux-Lantos V. Instituto de Biología y Medicina Experimental (IBYME-CONICET). ndigiorgio@ibyme.conicet.gov.arOur previous results have shown that in adult GABAB1KO male mice, which lack of GABAB receptors, Kiss1 mRNA expression was similar to wild type males in hypothalamic nuclei [arcuate (ARC) and anteroventral periventricular nucleus]. However, Kiss1 mRNA expression was markedly increased in extra-hypothalamic areas that are related to reproduction, such as medial amygdala (MeA), bed nucleus of the stria terminalis and lateral septum. To determine whether these characteristics were imprinted during development or could be mimicked in adulthood, here we studied the effects of the administration of a GABAB antagonist or a GABAB agonist on brain Kiss1 expression in adult mice. Adult Balb/c male mice were sc injected with CGP55845 (CGP, 1 mg/kg, a GABAB antagonist), Baclofen (Baclo, 5 mg/kg, a GABAB agonist) or saline as control (Sal) for 5 days, three times/day (8AM, 1PM, 6PM). Males were sacrificed at 3PM (after two injections on day 5). Serum samples and testes were obtained to determine hormone levels by RIA. Brains were frozen and 500µm slices were obtained on a cryostat. ARC and MeA were micropunched and Kiss1 mRNA expression was evaluated by qPCR (cyclophilin B, control gene). Baclo increased serum LH (p