Evaluación de cultivo celular en dispositivos de microfluídica para su aplicación en la producción de anticuerpos monoclonales

SIERRA-RODERO M.; KARP P.; HELGUERA G.; PEÑAHERRERA A. B.; ROSERO G.; BOURGUIGNON N.; PÉREZ M. S.; PAYÉS C.; LASORSA C.; LERNER B.

Córdoba

Congreso; 16º Congreso Internacional de Metalurgia y Materiales SAM-CONAMET; 2016

Asociación Argentina de Materiales

Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlledconditions, as is required for production of therapeutic recombinant proteins. To understand the optimalconditions for growth of cells amenable of recombinant protein expression in these devices, we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethylmethacrylate (PMMA) and polydimethylsiloxane (PDMS) microdevices, in the absence or presence of thecell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as theinfluence of the flow rate have also been tested, showing their great influence in cell adhesion and growth[1]. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells incontinuous or discontinuous flow [2]. Moreover, the optimal adhesion of cells was observed in the corners ofthe microchannels, as well as in wide channels and oval culture wells possibly due to the decrease in theshear stress in these areas. The microdevices with the oval culture wells design were utilized as models tostudy the distribution of cells infected with vesicular stomatitis virus (VSV). The distribution was evaluatedthrough the expression of green fluorescent protein (GFP). These results provide information to optimize architecture of microchannels for long-term culture of adherent cells in order to use microfludics devices asbioreactors for therapeutic monoclonal antibodies production, and biological activity of drugs in vitro.