TUMOR NECROSIS FACTOR ALFA (TNFα) EXPRESSION IN THE HIPPOCAMPUS FROM THE PROGENY OF EXPERIMENTAL DIABETES RATS. CHANGES BY EARLY INTAKE OF VEGETABLE OILS.

MAGRINI-HUAMAN, R.N; TAPIA, A.; IGHANI, M.; REY, M.; COIRINI, H.; FERESIN, G. E,

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuy; 2016

Sociedad de Biologia de Cuyo

Uncontrolledmaternal hyperglycemia during pregnancy produces alterations in the developingnervous system of the offspring. The aim of this study was to characterize theexpression of TNFα in the hippocampus of adult males (SD, 8 months old) bornfrom control mothers (CO) or mothers with experimental diabetes(streptozotocin, 30 mg /Kg iv; DO) that were submitted to an early dietarysupplementation with water (C), corn oil (Mz), extra virgin olive oil (OL) orpistachio oil (PS). Treatment were orally administered from day 2 to 62 (8μl/15g). TNFα expression was analyzed by western blot. Inmumoreactive bands of 51kDa (inactive, I) and 17 kDa (active, A) were quantified. Two way ANOVA showedsignificant changes (p<0.001) for I, in the condition (CO vs DO), treatment(C vs Oils) and interaction. For A only significant changes by treatment wasobserved (p<0.001). TNFα I showed DO<CO in C and Mz (p<0.05), DO>COfor OL and no changes for PS (p<0.05). The level of TNFαA was elevated forDO in C and OL (p<0.05), decreased in PS (p<0.05) and unchanged in Mz.These results indicate that an early supplementation with oils (rich in ω3)modulate the hippocampal TNFα expression 6 months after the treatment. The PSwas the most effective decreasing the expression of the TNFα active protein. (CONICET-PIP0243, PICTO/UCCuyo 2009-0158- CICITCAUNSJ-IDeA1400.0107/2012).