NITRIC OXIDE SYNTHASE (NOS) EXPRESSION IN THE HIPPOCAMPUS FROM THE PROGENY OF EXPERIMENTAL DIABETES MOTHERS. EFFECT OF THE INTAKE OF VEGETABLE OILS

MAGRINI-HUAMA;N, R.N; DELGADO, J.F.; TAPIA, A.; REY, M.; COIRINI, E.; VEGA, M.C.; IGHANI, M.; FERESIN, G. E,; COIRINI, H.

Ciudad Autonoma de Buenos Aires

Congreso; XVIII Jornadas Anuales Multidisciplinarias de la Sociedad Argentina de Biología; 2016

Sociedad Argentina de Biologia

Uncontrolled maternal hyperglycemia during pregnancy produces alterations in the developing nervous system of the offspring. In previous studies, we showed that pistachio oil (PS) increases exploratory activity in the open field test. The aim of this study was to: 1) Evaluate the expression of hippocampal NOS in adult rats (SD, male/female, 8 months old) born from control mothers (CO) or mothers with experimental diabetes (streptozotocin, 30 mg /Kg iv; DO) and 2) Study the effect of early dietary supplementation with corn oil (MZ), extra virgin olive oil (OL) and PS. The oils were administered from day 2 to 62 of age (8μl /15g). NOS expression was analyzed by western blot. In males NOS level was significantly higher in the DO than in CO (ANOVA, p <0.001) with or without oil supplementation. CO highest expression was observed with PS (p <0.001), whereas in DO it was found with OL (p <0.01), no significant differences between CO-PS and DO-PS (p = 0.871) was found. The results were similar in females, higher level of NOS in DO than CO (p <0.001) and OL and PS increased NOS expression in CO and DO (p <0.01) respectively. These studies indicate that early supplementation with oils rich in ω3 to DO does not decrease the hippocampal expression of NOS. In addition, it seems to be some sex dependent responses to the treatments. (CONICET-PIP0243; PICTO/UCCuyo2009-0158; CICITCA UNSJ-IDeA1400.0107/2012).