ALTERED NEUTROPHIL CHEMOTAXIS IN THE PROGENY OF DIABETIC DAMS

DELGADO, J.F.; CAÑELAS, A.; FERESIN, G. E,; MAGRINI-HUAMA;N, R.N; RUIZ, B.; RAMIS PELEGRINA, E.B.; LARREA, C.; TAPIA, A.; COIRINI, H.

Congreso; XLI Reunion Cientifica Anual de la Sociedad Argentina de Investigación Clínica,; 2016

Sociedad Argentina de Investigacion Clinica

The uncontrolled hyperglycemia during pregnancy,increases oxidative stress on the progeny however, early treatment could reduceor reverse the produced effects of the reactive species generated. Theinclusion in the diet of monounsaturated fatty acids has shown a positiveeffect in this regards. The aim of this study was to analyze the action ofextra virgin olive (OL) and pistachio oil (PS) using corn oil (MZ) as control,on theinflammatory response at the peripheral level in adult offspring from mothers with experimentaldiabetes (DO) or from control mothers (CO). Rats were supplemented with oilfrom day 2 to 62 (8μl/15g body weight). At 4months old, neutrophil chemotaxis in agarose and the expression of CD44 (anadhesion molecule expressed by these cells) by flow cytometry, were evaluated.Chemotaxis studies showed significant changes between CO/DO, indicating thatthe altered intrauterine conditions affect neutrophil migration regardless ofthe treatment or gender. The DO showed 35% higher migration than the CO(p<0.05). Sexual differences was also observed (p<0.05), femaleneutrophils showed 23% greater mobility than those from male rats. Howevertreatment with oils showed no significant differences among groups. Regardingthe expression of CD44, no significant changes between CO/DO groups or bygender were observed, but PS-treated animals had a 22% reduction (p<0.05)compared to MZ. Studies indicated that the experimental diabetes duringpregnancy altered the migratory ability of neutrophils but not the CD44expression. The increase in chemotaxis may be probably related to increasedinsulin production because DO become glucose intolerant. On the other hand thedecrease in CD44 by PZ is reflecting a greater protective action of this oilthan OL or MZ. (08CM13 UCCuyo, PIP0243CONICET, Fund. Rene Barón).