FGFR2 modulates the metastatic potential of breast cancer cells

LANARI C; LÜTHY IA; RODRÍGUEZ R; PÉREZ PIÑERO C

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

We have previously shown that FGF2 activates FGFR2 and progesterone receptors (PR) increasing the proliferation of breast cancer cells suggesting that the activation of the FGF-FGFR axis might be important in the acquisition of hormone independence. It has been reported that FGF2 induces RUNX2 expression and highlevels of RUNX2 were detected in mammary carcinomas which acquired a hormone independent phenotype. The aim of this study was to evaluate the effect of constitutively active FGFR2 on a human breast cancer cell line. IBH-6 cells express estrogen receptor (ER), PR and FGFR 1-4, RUNX2 and they grow in vivo in NSG mice without hormone supply giving rise to invasive carcinomas. We havealready shown that IBH-6 cells with silenced FGFR2 give rise to smaller tumors, showed a less aggressive phenotype, a lower proliferation index and a decrease in CCND1 and RUNX2 expression compared with control tumors. In this study we transfected IBH-6 cells with a constitutively active FGFR2 (R2CA) or with a control plasmid. Control cells showed a higher proliferation index in vitrowhen treated with FGF2 (p