IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
AKT1 and AKT2 EXPRESSION AND LOCALIZATION IN HUMAN BREAST CANCER SAMPLES
Autor/es:
MARÍA MAY; D. L. KAEN; VIRGINIA NOVARO; MARÍA CECILIA PERRONE; MARÍA LAURA POLO; EDUARDO RICHARDET; MARINA RIGGIO; MARÍA JIMENA RODRIGUEZ; CLAUDIA LANARI
Lugar:
Buenos Aires
Reunión:
Simposio; Ganando la guerra contra el cáncer; 2016
Institución organizadora:
Programa RAICES
Resumen:
Deregulation of PI3K/AKT/mTOR pathway is associated with breast cancer progression and resistance to therapy. Nowadays, there are clinical trials that combine endocrine treatments with selective inhibitors of this pathway, although they don?t distinguish between AKT isoforms (AKT1, AKT2 and AKT3) and their specific functions.Previous results in our group showed that in human breast cancer cell lines positive for homone receptors (HR+), AKT1 promotes cell proliferation by regulating Cyclin D1 and S6, and has an anti-migratory function by inhibiting β1-Integrine and downstream proteins; whereas AKT2 stimulates cell migration by regulation of Actin polymerization and Vimentin levels. Moreover, in xenografts of these cell lines we observed that silencing AKT1 or overactivating AKT2 generates a more aggressive tumor phenotype and lung metastasis.With the aim of assessing the prognostic value of AKT isoforms expression and localization in patients with advanced HR+ breast cancer, we evaluated the levels of AKT1 and AKT2 by immunohistochemistry using tissue microarrays (TMAs) with 46 samples and searched for associations with different clinical and histopathological parameters.We found a positive correlation (p=0,0407) between nuclear AKT1 expression and KI-67 levels, a negative correlation between cytoplasmatic AKT2 levels and patients? age at diagnosis (p=0,0146), and a positive association (p