IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
Autor/es:
ANA ROMAROWSKI; LIS DEL C. PUGA MOLINA; ALBERTO I. DARSZON; CLAUDIA SÁNCHEZ-CÁRDENAS; CLAUDIA L. TREVIÑO; BUFFONE MG.; HÉCTOR V. RAMÍREZ-GÓMEZ; ARTURO HERNÁNDEZ-CRUZ
Reunión:
Simposio; Frontiers in Reproduction. Nineteenth Annual Symposium.; 2016
Resumen:
During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in the mammalian fertilization. It was long believed that this event occurred upon sperm interaction with the zona pellucida. However, recent results demonstrated that most of mouse sperm undergo exocytosis prior to interacting with the egg extracellular matrix. In this scenario, Progesterone (Prog) has emerged as a potential physiological inducer of the AR. This steroid produced by cumulus and granulosa cells has been associated with various physiological processes in sperm, including motility, chemotaxis, capacitation and AR. It is established that an increase in intracellular Ca2+ ([Ca2+]i) is necessary for AR to occur. Since Prog promotes an a [Ca2+]i increase, it becomes necessary to simultaneously the exocytosis and [Ca2+]i changes in a single sperm. In this context, we studied the spatio-temporal correlation between the changes in [Ca2+]i and AR in single mouse spermatozoa in response to Prog. We found that Prog stimulates an [Ca2+]i increase in a statistically significant percentage of sperm (p