IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of Wnt/bETA-catenin signal transduction pathway in rat follicular development and luteal function
Autor/es:
PAULA ACCIALINI; GRISELDA IRUSTA; DIANA BAS; FERNANDA PARBORELL; DALHIA ABRAMOVICH; MARTA TESONE
Lugar:
Mendoza
Reunión:
Workshop; International Workshop in Neuroendocrinology (WNE); 2015
Resumen:
Role of Wnt/beta-catenin signal transduction pathway inrat follicular development and luteal functionMarta Tesone Sr., Paula Accialini, Griselda Irusta,Fernanda Parborell and Dalhia AbramovichIBYME-CONICET, Buenos Aires, ArgentinaThe evolutionarily conserved Wnt/beta-catenin signal transductionpathway controls many biological processes. The objectiveof this study was to determine the role of this signaling pathwayin follicular development and luteal function. To this purposePMSG-eCG gonadotropin- prepubertal treated female rats wereinjected with a Wnt inhibitor (XAV939, 5ug/ovary, Wnt-I group)or vehicle (DMSO, Control group) into the bursa of both ovariesthe day of hCG administration.Two days after hCG and Wnt-Ior vehicle injection, blood samples and ovaries were collected.Ovarian function was evaluated by measuring serum progesterone(P4) (RIA), steroidogenic- regulators protein levels bywestern blot, apoptotic parameters and ovarian structures atdifferent stages of development. The levels of P4 significantlydecreased after Wnt inhibitor administration. Corpora lutea (CL)were isolated by microdissection and steroidogenic proteinregulators were measured. StAR levels significantly decreased inthe Wnt-I group in comparison with the Control group, whereasthe levels of P450scc or 3 beta HSD enzymes did not change.IHC studies showed that luteal cells exhibited high stainingfor active Caspase3 in the Wnt-I group in comparison with theControl. Histological studies showed a tendency to a decrease inthe percentage of corpora lutea and an increase in antral folliclesin the Wnt-I group. In conclusion, the inhibition of Wnt pathwayappears to produce a decrease in P4 serum levels associated toa decrease in StAR levels in CL, an increase in active Caspase3expression and a tendency to inhibit luteinization.O