Role of Galectin 1 in the pathogenesis of Inflammatory Bowel Diseases

RODRIGO PAPA GOBBI; ANDRES ROCCA; ALICIA SAMBIELLI; RENATA CURCIARELLO; MARTIN YANTORNO; GUSTAVO CORREA; LUCIANO G. MOROSI; KARINA V. MARIÑO; MARTA A. TOSCANO; CECILIA MUGLIA; GABRIEL A. RABINOVICH; GUILLERMO H. DOCENA

Buenos Aires

Congreso; First International Joint Meeting LASID-SAI-FAIC; 2015

Latin American Society for Immunodeficiencies (LASID), the Argentinean Society for Immunology (SAI) and the French Society for Immunology (FAIC)

Background: Inflammatory bowel diseases (IBD) are chronic and relapsing intestinal inflammatory disorders. Galectin-1 (Gal-1) is a lectin with anti-inflammatory properties and therapeutic potential in immune-mediated disorders. In this study we investigated the role of Gal-1 in the colonic mucosa of patients with IBD.Methods: Intestinal specimens from control (HC) (n=46) and IBD patients (n=82) were studied by RT-qPCR, immunoblotting, immunohistochemistry and ELISA for Gal-1 production and flow cytometry for detection of Gal-1-specific ligands in lamina propria T cells (LPTC). Gal-1 functional assays were performed by flow cytometry (Annexin V) and TUNEL assay.RESULTS: We found Gal-1 mRNA expression in colonic inflamed areas (p