Endometrial growth inhibition by natural compounds evaluated as new therapeutic agents for endometriosis

FERELLA LUCIANA; BASTON JUAN IGNACIO; BILOTAS MARIELA; SINGLA JOSÉ JAVIER; GONZALEZ ALEJANDRO; OLIVARES CARLA; MERESMAN GABRIELA

Congreso; Endometrial growth inhibition by natural compounds evaluated as new therapeutic agents for endometriosis; 2015

Study question: The objective of our study wasto evaluate the effect of Wogonin (WG), an active constituent of Chinese HerbalMedicine, and two of the main antioxidant compounds found in rosemary extract:Carnosic Acid (CA) and Rosmarinic Acid (RA) on the development of experimentalendometriosis both in vitro and in vivo.Summaryanswer: All natural compounds evaluated exerted an inhibitory effect onendometrial growth or endometriosis development. CA, RA and WG inhibitedsignificantly cell proliferation in human endometrial stromal cell line T-HESC,and in primary cultures. In a murine model,these compounds diminished significantly the development of endometriotic-likelesions, reducing considerably its size.What is known already: Endometriosis is a chronic disease, with high levelsof recurrence after standard medical therapy. The past few years haveseen a renewed interest in the use of natural compounds due to their advantages:long term administration with minimal side effects. WG isknown to inhibit proliferation and induce apoptosis in many kinds of cancerouscells. Similarly, principal components of rosemary (Rosmarinus officinalis) leaves, CA and RA, have been reported tohave antioxidant and antitumor activities.Study design,size, duration: Primary cultures of stromal cells from eutopic endometrial biopsies ofpatients with endometriosis and controls, and the T-HESC cell line were incubated withWG, CA or RA for 24-h. Endometriosis was surgically induced in BALB/c mice.Animals received WG, CA or RA daily from post-surgical day 14 until day 28. Participants/materials, setting, methods: Cell proliferation was evaluated by MTS assay inprimary and T-HESC cell cultures. Mice were randomly assigned to different treatmentgroups: RA-1mg/kg (n=11); RA-3mg/kg (n=10); CA-2mg/kg (n=11); CA-20mg/kg(n=10); WG-20mg/kg (n=12) and Control (n=8). Animals received CA or RA daily byintraperitoneal injection or WG daily by gastric gavage.Mainresults and the role of chance: WG 40, 80 and 160 µM; CA 10, 12.5and 25 µg/ml and RA 25, 50 and 100 µg/ml significantly inhibited cellproliferation in T-HESC cell line (p