Los tóxicos ambientales endothall y nonilfenol inducen la reacción acrosómica vía la proteína cinasa a en espermatozoides de ratón.

GALLARDO LM; PUGA MOLINA L; BUFFONE MG; MORENO RD

Antofagasta, Chile

Congreso; XXI Reunión Anual de la Sociedad Chilena de Reproducción y Desarrollo; 2015

Sociedad Chilena de Reproducción y Desarrollo

It has shown that environmental toxicants, could affect the reproductive outcome, but there is no study about how these compunds could disturb the fertilization processes. In this work, we select two environment contaminants, Endothall (E), a widely used pesticide, which inhibits the phosphatase PP2A and nonylphenol (NP), a xenoestrogen that mimic the estrogen effect. Our goal was to determine whether E or NP induce the AR, by regulating activation of protein kinase A (PKA). To this end, mouse epididymal caudal spermatozoa were recovered with or without the PKA inhibitor H89 and then, incubated with E, NP, or progesterone (P4) and the calcium ionofore A21187 as positive controls, in capacitating (CM) or non capacitating conditions (NCM). Inicially, we study PP2A (target of Endothall) sperm localization in mouse sperm, and later, we observe that Endothall and NP induce the AR in mice sperm through a PKA pathway by different assays, in a concentration range found in the environment. In conclusion, our findings show that E and NP induce the AR in mouse sperm, sugesting that this compounds,also, could disturbed the fertilization process.