IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IDENTIFICATION OF CRISP PROTEINS IN THE DOG FOR THEIR POTENTIAL USE IN IMMUNOCONTRACEPTION
Autor/es:
BRUKMAN N; LA ROSA I; DE SALVO MN; CICUTTIN GL; DA ROS VG; LENCINAS OE; CUASNICÚ PS
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
There is a growing need to reduce the dog population
levels as a means to control diseases transmitted to human (zoonosis). Based on
previous results from our group supporting epididymal CRISP1 (Cysteine-Rich Secretory
Protein 1) as a good immunocontraceptive target, the aim of this work has been
the identification of a homologue CRISP protein in the dog that could be used
for immocontraceptive development in this species. Using degenerated primers against conserved
CRISP motives, we obtained a RT-PCR band of the expected size (aprox. 120 bp)
from epididymal but not testicular samples.DNA sequencing of this band showed a
high(>80%)homology to CRISP proteins. Western blotting results using
antibodies against different CRISP proteins revealed the presence of 25 kDa
band in epididymal but not testicular extracts when an anti-mouse CRISP3
antibody was used. As expected for a CRISP molecule, theepididymal band shifted
itselectrophoretic mobility under reducing conditions. Moreover, the protein
precipitates by exposure to a 50% saturated ammonium sulfatesolution as
previously observed for rodent CRISP1. Altogether, these observations support
the identification of a CRISP-like molecule in the dog epididymis which is at
present being further characterized for future purification and functional
studies.