IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A novel spherical treadmill apparatus for the head-fixed behaving rat
Autor/es:
M. M. MIGUELEZ FERNÁNDEZ; ARIEL BURMAN; ALFREDO I. MARTÍNEZ CÁCERES; B. SILVANO ZANUTTO
Lugar:
Huerta Grande. Cordoba
Reunión:
Congreso; XXIX CONGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS; 2014
Institución organizadora:
SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS
Resumen:
P84.-A
novel spherical treadmill apparatus for the head-fixed behaving rat
Anabel M. M. Miguelez
Fernández1°, Ariel Burman2°, Alfredo I. Martínez Cáceres2°, B. Silvano
Zanutto1°,
Sergio E. Lew2°
1°
Instituto de Biología y
Medicina Experimental (IByME - CONICET), Laboratorio de Biología del
Comportamiento,
Ciudad de Buenos Aires,
Argentina.; 2°
Instituto de Ingeniería
Biomédica (IIBM-UBA), Facultad de Ingeniería,
Universidad de Buenos Aires,
Ciudad de Buenos Aires, Argentina
anabelmariamercedes@gmail.com
We
introduce a novel training apparatus for rodents that allows animals to perform
motor responses
while
being head fixed.
This
apparatus was developed for the purpose of recording extracellular neuronal
activity in the motor
cortex
of an animal while it performs a motor task. It consists of a freely rotating
spherical treadmill
and an
iron structure where animals are head fixed. Once secured to the structure,
animals are placed
over
the treadmill with their limbs on the sphere so they can walk in any direction.
Head fixation is
achieved
by an x-shaped aluminum piece that is surgically implanted on the skull.
Movements
of the animal are measured as rotations of the spherical treadmill. Sphere
speed and rotation
direction
are detected with a 60fps video camera, using custom designed software. The
software also
allows
a wide control of training parameters and performs data storage for posterior
analysis.
Here we
report training a Long Evans rat in an operant conditioning task where walking