IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
?Role of wnt/bcatenin signal transducción pathway in rat follicular development and luteal function?.
Autor/es:
ACCIALINI P, BUSSO D, BAS D, PARBORELL F, ABRAMOVICH D, TESONE M
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornada Anual de la Sociedad Argentina de Biología (SAB); 2014
Institución organizadora:
Sociedad Argentina de Biología (SAB)
Resumen:
The evolutionarily conserved Wnt/b-catenin signal transduction pathway
controls many biological processes. The objective of this study was to determine the role of this signaling pathway in follicular development and
luteal function. To this purpose PMSG-eCG gonadotropin- prepubertal treated
female rats were injected with a Wnt inhibitor (XAV939, 5ug/ovary, Wnt-I group) or vehicle
(DMSO, Control group) into the bursa of both ovaries the day of hCG
administration. Two days after hCG and Wnt-I or vehicle injection, blood samples and
ovaries were collected. Ovarian function was evaluated by measuring serum
progesterone (P4) (RIA), steroidogenic regulators protein levels and
ovarian structures at different stages of development. The levels of P4
significantly decreased after Wnt inhibitor administration. Corpora lutea (CL)
were isolated by microdissection and steroidogenic protein regulators were
measured by Western blot. StAR levels significantly decreased in the Wnt-I group in comparison with
the Control group, whereas the levels of P450scc
or 3 beta HSD enzymes
did not change. Histological studies showed a tendency to a
decrease in the percentage of corpora lutea and an increase in antral follicles
in the Wnt-I group. In
conclusion, the
inhibition of Wnt pathway appears to produce a decrease in P4 serum levels associated to a decrease in StAR levels in CL and a tendency to inhibit
luteinization