IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Local Sphingosine 1-phosphate (S1P) administration affects follicular/luteal development and enhances vascular integrity in ovarian hyperstimulation syndrome (OHSS)
Autor/es:
DI PIETRO M, SCOTTI L, PASCUALI N, BAS D, IRUSTA G, TESONE M, ABRAMOVICH D AND PARBORELL F
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornada Anual de la Sociedad Argentina de Biología (SAB); 2014
Institución organizadora:
Sociedad Argentina de Biología (SAB).
Resumen:
OHSS remains the most serious complication of
gonadotropin treatment. Despite advances in prediction and management of OHSS,
complete prevention has not been possible yet. The main clinical components are
marked enlargement of the ovaries, with luteal and hemorrhagic cysts, and an
excessive fluid shift. This shift is caused by increased vascular permeability
in response to hormonal stimulation. S1P is an active lysophospholipid that
exerts pleiotropic effects including cell proliferation, cell survival, cell
migration and cell adhesion. Previously, we observed decreased S1P levels in
ovarian follicular fluid (FF) from OHSS patients. The aim of this study was to
investigate the effects of S1P administration on the follicular and luteal development,
cystic structures, peri-endothelial cell area, S1P1/3 receptors, sphingosine
lyase (Sly)and kinase (Sk), n-cadherin, nectin-2 and occludin levels in ovaries
from an OHSS rat model. Sprague Dawley rats were injected prepubertal eCG (10
IU) and 48 hours later with hCG (10 IU) (Control). The OHSS group was injected
with eCG (50 IU / day) for 4 consecutive days and 24 hours later with hCG (25
IU). The group OHSS + S1P received S1P (5ul/ovary; 1mM) under the bursa of one
ovary in the day of hCG inyection. The rats were sacrificed 24 h. post hCG.
Ovaries were isolated for histological sections to detect α-actin (cell
periendothelial marker) by IHQ and to isolate proteins by western blot. S1P
decreased significantly the percentage of corpora lutea and cyst
structures compared to OHSS group alone (p<?and
p<?., respectively). No significant changes were observed in the percentage
of preantral and early antral follicles. S1P treatment did not affect
peri-endothelial area. S1P injection increased N-cadherin and occludin levels whereas
nectin-2 levels did not change compared to OHSS group without treatment. In
OHSS group, the levels of Sly and Sk were unaffected by S1P. In addition, S1P
increased levels of its own receptor, S1P1, regarding OHSS group alone. In
conclusion, our findings indicate that ovarian S1P administration prevents the
early onset of OHSS and decreases its severity in rats.