Natural compounds evaluated as new therapeutic agents for the treatment of endometriosis

FERELLA L; BASTÓN JI; BILOTAS M; GONZALEZ A; OLIVARES C; MERESMAN GF

Jornada; XVI Jornadas de la Sociedad Argentina de Biología; 2014

Objective: To evaluate the effect of Wogonin, an active constituent of Chinese Herbal Medicine; two of the main antioxidant compounds found in rosemary extract: Carnosic Acid (CA) and Rosmarinic Acid (RA); and 1α,25-dihidroxivitaminD3 (VD3) in vitro and in vivo, in experimental endometriosis. These compounds demonstrated antiproliferative properties in several cancer models. Design: Stromal endometrial cells derived from endometriosis patients and the T-HESC cell line were cultured with Wogonin, CA or RA for 24-h, or VD3 for 48-h. Endometriosis was surgically induced in BALB/c mice. Animals were assigned randomly to different groups: RA-1mg/kg; RA-3mg/kg; CA-2mg/kg; CA-20mg/kg; Sham and Control. Materials and Methods: Cell proliferation was evaluated by MTS assay in primary and T-HESC cell cultures. Mice received the compounds daily by intraperitoneal injection from post-surgical day 14 and continued until day 28. After treatment, animals were sacrificed, the abdomen was opened, lesions were counted, measured and their volume calculated. Results: Wogonin 40, 80 and 160 µM; CA 10, 12.5 and 25 µg/ml and RA 25, 50 and 100 µg/ml significantly inhibited cell proliferation in T-HESC cell line (p