In vitro and in vivo endometrial growth inhibition by natural compounds evaluated as new therapeutic agents for endometriosis.

FERELLA LUCIANA; BASTON JUAN IGNACIO; BILOTAS MARIELA; SINGLA JOSE JAVIER; OLIVARES CARLA; MERESMAN GABRIELA

San pablo

Congreso; 12th World Congress on Endometriosis; 2014

IN VITRO AND IN VIVO ENDOMETRIAL GROWTH INHIBITION BY NATURAL COMPOUNDS EVALUATED AS NEW THERAPEUTIC AGENTS FOR ENDOMETRIOSIS. Luciana Ferella, Juan Ignacio Bastón, Mariela Bilotas, José Javier Singla, Carla Olivares, Gabriela Meresman. Objective: To evaluate the effect of Wogonin, an active constituent of Chinese Herbal Medicine; two of the main antioxidant compounds found in rosemary extract: Carnosic Acid (CA) and Rosmarinic Acid (RA); and 1α,25-dihidroxivitaminD3 (VD3) in vitro and in vivo, in experimental endometriosis. These compounds demonstrated antiproliferative properties in several cancer models. Design: Stromal endometrial cells derived from endometriosis patients and the T-HESC cell line were cultured with Wogonin, CA or RA for 24-h, or VD3 for 48-h. Endometriosis was surgically induced in BALB/c mice. Animals were assigned randomly to different groups: RA-1mg/kg; RA-3mg/kg; CA-2mg/kg; CA-20mg/kg; Sham and Control. Materials and Methods: Cell proliferation was evaluated by MTS assay in primary and T-HESC cell cultures. Mice received the compounds daily by intraperitoneal injection from post-surgical day 14 and continued until day 28. After treatment, animals were sacrificed, the abdomen was opened, lesions were counted, measured and their volume calculated. Results: Wogonin 40, 80 and 160 µM; CA 10, 12.5 and 25 µg/ml and RA 25, 50 and 100 µg/ml significantly inhibited cell proliferation in T-HESC cell line (p