IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Platelet-Derived Growth Factor BB and DD and Angiopoietin1are altered in follicular fluid from Polycystic Ovary Syndrome patients.
Autor/es:
ABRAMOVICH DALHIA; SCOTTI LEOPOLDINA; IRUSTA GRISELDA; PAZOS CAMILA; DE ZUÑIGA IGNACIO; BISIOLI CLAUDIO; PARBORELL FERNANDA; TESONE MARTA
Lugar:
Chicago
Reunión:
Congreso; ICE-ENDO 2014; 2014
Institución organizadora:
Endocrine Society
Resumen:
Polycystic Ovary Syndrome (PCOS) is the most common endocrinological
pathology among women of reproductive age. Among other diverse features, PCOS
is characterized by abnormalities in ovarian angiogenesis. While VEGF is the
main angiogenic factor that promotes endothelial cell migration and
proliferation, there are other angiogenic factor families involved in
angiogenic process. Among these families are the Angiopoietin (ANGPT) and PDGF
families, both involved in vessel stabilization and vascular permeability. It
is known that follicular fluid (FF) levels and ovarian expression of VEGF are
increased in PCOS patients. However, there are no studies analyzing other angiogenic
factors in ovaries from PCOS women. Our hypothesis was that besides VEGF, other
angiogenic factors are dysregulated in the ovaries from these patients, leading to altered angiogenesis
and vascular permeability. Therefore, in this study we examined the levels of
ANGPT1, ANGPT2, their soluble receptor TIE2 (sTIE2), PDGFBB and PDGFDD proteins
in FF from PCOS (n=19) and control (n=27) patients undergoing assisted
reproductive techniques. In addition, we analyzed the effect of FF from these
patients on endothelial adherens and tight junction protein expression. We
found a significant increase in the levels of ANGPT1 in PCOS patients (control:
201.1±23.1 pg/ml; PCOS: 516.4±81.8 pg/ml; p<0.0001) with no changes in the
levels of the two soluble antagonists ANGPT2 and sTIE2. PDGFBB and PDGFDD were
decreased in PCOS patients (PDGFBB: control: 1.29±0.15; PCOS: 0.81±0.05
arbitrary units; p<0.01) (PDGFDD: control: 2.37±0.14; PCOS: 1.94±0.07
arbitrary units; p<0.05). The levels of claudin 5 were significantly
increased in endothelial cells incubated for 24h with PCOS follicular fluids
compared to control FF (control: 2.05±0.13; PCOS: 3.50±0.52 arbitrary units; p<0.05).
The levels of p-VE-cadherin, p-beta-catenin and ZO1 were no different in cells
incubated with control and PCOS FF at any time point analyzed. These results
suggest that the alterations observed in the ANGPTs and
PDGFs levels may prevent VEGF-induced vascular leakage in the ovary at least in
part by regulating endothelial cell junction proteins. Restoration of the
levels of angiogenic factors may provide new insights to PCOS treatment and
OHSS prevention in PCOS women.