Targeting the galectin-1-mediated angiogenesis at peritoneal level is crucial to constrain the progress of endometriosis

BASTÓN, JUAN IGNACIO; BARAÑAO, ROSA INÉS; BILOTAS, MARIELA; CROCI, DIEGO; RABINOVICH, GABRIEL ; MERESMAN, GABRIELA

San Pablo

Congreso; 12th World Congress on Endometriosis; 2014

World Endometriosis Society

The endogenous lectin, Galectin-1 (Gal-1), plays a pivotal role in the vascular development and consequently growth of endometriotic lesions. A targeted blockade of Gal-1 at peritoneal level was performed with the aim to evaluate an experimental approach that could shed light on a future potential therapeutic strategy for the endometriosis. Endometriosis was surgically induced in C57BL/6 mice. Targeted blockade of Gal-1 at peritoneal level was carried out with a neutralizing anti-galectin-1 monoclonal antibody. The number and size of developed endometriotic lesions, besides the relative vascularized area and the peritoneal content of proangiogenic cytokines were assessed. Endometriotic-like lesions were induced in C57BL/6 by autologous transplantation of endometrial tissue to bowel mesentery. Six mice were injected intraperitoneally 3 times for week with 15 mg/kg of the neutralizing anti-Gal-1 antibody F8.G7, and 6 mice with the IgG isotype antibody, from second to fourth week post endometriosis induction. Despite there was no change in the number of developed endometriotic lesions between groups, notably, the peritoneal blockade of Gal-1 resulted in a significant reduction of lesions size in anti-Gal-1 treated mice compared to controls (P