. In vitro study of two natural compounds for endometriosis treatment: resveratrol and EGCG.

RICCI A; OLIVARES C; ALVARADO-DIAZ C; GONZALEZ RAMOS R; MERESMAN G; BARAÑAO RI

San Pablo

Congreso; 12th World Endometriosis Congress; 2014

World Endometriosis Society

Objective: We study the in-vitro effect of two natural polyphenols, resveratrol and EGCG on endometrial cells proliferation and their possible molecular mechanism of action through the NF-кB pathway. Our previous studies shown that both compounds have a strong antiproliferative and proapoptotic activity both in vivo and in vitro in experimental endometriosis. Design: Endometrial carcinoma cell line (ECC-1) was cultured according to standard protocols in RPMI 5% fetal bovine serum. After 48 hours, cells were harvested and 24 hours later were stimulated with different doses of resveratrol (50 and 100µM) or epigallocatechin gallate (20 and 40µM) for 24 hours. Materials and methods: Cell proliferation was assessed using the MTS reduction kit. Nuclear and cytoplasmic extractions were performed by NE-PER kit. The activation of NF-кB (p65) was evaluated using the DNA-binding-Elisa TransAM kit and the levels of total IкBα in citoplasmic fractions were assessed by Western Blot. Results: We found that both resveratrol and epigallocatechin gallate induced an inhibition of proliferation in ECC-1 (p