IDENTIFICATION OF HEPARAN SULFATE IN HUMAN OOCYTES AND PRELIMINARY EVIDENCE ON ITS POSSIBLE ORIGIN

ROMANATO M ; FARRANDO B ; JULIANELLI V ; CALVO L ; CALVO JC

Chascomús

Jornada; XV Jornadas Anuales de la SAB; 2013

SAB

Oocyte decondensing ability varies with oocyte maturation. We have proposed that HS present in ooplasm acts as human sperm decondensing agent in vivo. The aims of this study are: 1) to determine the presence of HS at different stages of oocyte maturation and 2) to characterize GAG synthesis in mature (MII) oocytes, Cumulus Cells or Cumulus Oocyte Complex. 1) Immunocytochemistry of Germinal Vesicles, MII and CC revealed that HS is present in MII (65510±1582 Arbitrary Units vs 8579±742 AU (control), t-test, p