Participación de KISS1 en las alteraciones reproductivas de hembras RGABAB1KO.

DI GIORGIO N, ; LÓPEZ P, ; SEMAAN S,; BETTLER B, ; LIBERTUN C, ; KAUFFMAN A, ; LUX-LANTOS V

CABA, Buenos Aires

Jornada; Primer encuentro Rioplatense de biologia-XIV jornadas anuales de la Sociedad Argentina de Biología.; 2012

SAB

Kisspeptin, encoded by Kiss1 and synthesized in the AVPV and ARC hypothalamic nuclei, stimulates GnRH neurons. GABA, through GABAA and GABAB receptors (GABABRs), also regulates reproduction. Adult female GABAB1RKO mice have altered GnRH levels and pulsatility, and compromised fertility. However, the interaction between GABABRs and Kiss1 neurons (Kiss1n) is unknown. We studied Kiss1 participation in the reproductive impairments of GABAB1RKOs and the role of sexual steroids therein. We assessed in WT and GABAB1RKO adults the GABAB1R co-localization in AVPV Kiss1n (double-label in situ hybridization (ISH), only females), Kiss1 mRNA in AVPV and ARC (ISH and qPCR), estrogen receptor alpha (ERa) mRNA in anterior hypothalamus (AH), including AVPV (qPCR), and estradiol and testosterone serum and gonad contents (RIA). Nearly all Kiss1 neurons (~98%) co-express GABAB1R in the AVPV. Kiss1 mRNA, by ISH and qPCR, was similar between genotypes in the AVPV and ARC. Sex steroids were similar between genotypes. However, ERa in AH was higher in GABAB1RKOs than in WTs. Thus, reproductive disruption in GABAB1RKO females is not due to abnormal Kiss1 expression in AVPV or ARC. However, this phenotype might be influenced by increased sensitivity to estradiol in AH. (CONICET, UBA, ANPCYT, ISN-CAEN)