Neonatal Exposure to Bisphenol A Alters Steroid Hormones and GNRH-I Content in the Ovary of Adult Rats.

BOURGUIGNON N S; BONAVENTURA MM; LUX LANTOS V; LIBERTUN C

Houston, TX

Congreso; The Endocrine Society's 94th Annual Meeting; 2012

The Endocrine Society (USA).

Bisphenol A (BPA), is an endocrine disruptor with multiple effects; it behaves as an estrogen agonist and/or antagonist and as an androgen antagonist. In previous works we described the effects of BPA on the hypothalamic‐pituitary‐gonadal axis in female rats; we found that neonatal exposure to BPA was associated in adulthood with high serum estradiol (E2) and testosterone (T) levels, reduced serum progesterone (P), and altered in vitro hypothalamic GnRH secretion. Animals exposed to BPA also had altered ovarian morphology (1, 2, 3). Following this line, here we study the ovarian content of E2, P4, T and GnRH‐I. Female Sprague‐Dawley rats were injected subcutaneously, daily from the day of birth to postnatal day (PND) 10 with BPA in castor oil at 50 μg/50μl (BPA50), 500 μg/50μl (BPA500), or castor oil (Control) (1). Rats were sacrificed in the morning of estrus at 110‐120 days of age. The ovarian content of E2, P, T and GnRH‐I were evaluated by RIA. Results were expressed as means SE. Data were analyzed by one‐way analysis of variance and were considered significant when p