IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of endometriosis (EDT) on follicle development and apoptosis in the ovary in a rat model.
Autor/es:
BILOTAS M.; OLIVARES C.; ABRAMOVICH D; MERESMAN GF; BARAÑAO RI
Lugar:
Buenos Aires
Reunión:
Congreso; XIV Jornadas de la Sociedad Argentina de Biología - Primer encuentro rioplatense de biología; 2012
Institución organizadora:
Sociedad Argentina de Biología. Sociedad Uruguaya de biociencias
Resumen:
Different mechanisms have been proposed to explain EDT associated infertility including alterations in follicular and peritoneal environment, in folliculogenesis and in granulosa cells function. The objective of this work was to evaluate the effect of EDT in follicle development and in apoptosis in the ovary. EDT was surgically induced in female Sprague Dawley rats. One month after surgery animals were sacrificed at proestrus. EDT-like lesions were removed, fixed, sectioned and stained to confirm the development of EDT. Both ovaries were excised. One was fixed, sectioned at 50 um intervals and stained, and follicles at different stages were counted. Total protein extracts from the other ovary were used to determine the expression of pro- and anti-apoptotic proteins by Western blot. The number of total ovarian structures was reduced in EDT animals compared to the Sham group (p