Correlation between FGFR-2/ STAT-5 and RUNX-2 expression in breast cancer.

MAY, M; ROJAS, P; SEQUEIRA, G; MARTINEZ, P; GONZALEZ, P; GASS, H; LANARI, C

Capetown

Congreso; IAP 2012 XXIX International Congress in Cape Town, South Africa; 2012

THE INTERNATIONAL ACADEMY OF PATHOLOGY

Correlation between FGFR-2/ STAT-5 and RUNX-2 expression in breast cancer. May M. 1*, Rojas P. 1, Sequeira G. 1, Martinez P. 2, Gonzalez P. 2, Gass H. 2, Lanari C1. 1Laboratory of Hormonal Carcinogenesis, IByME-CONICET, Buenos Aires, Argentina 2General Pacheco Hospital, Buenos Aires, Argentina *drmariamay@gmail.com Introduction: We have recently reported that FGFR2 is involved in FGF2-induced progesterone receptor (PR) activation in a murine breast cancer model, and that STAT5 is involved in the interaction between FGFR2 and PR. In addition, RUNX2 is one of the up regulated genes observed by gene array analysis in the tumors which have acquired a hormone independent growth. It has been reported that a polymorphism in the FGFR2 gene is related to sporadic ER- and PR-positive breast cancer risk and that the SNPs involved alter the binding affinity for the transcription factors OCT-1/RUNX2 and EBP-β. Taking into account that FGF2 activates RUNX2 and that a physical interaction between STAT5 and RUNX2 has also been reported, we decided to investigate the expression of FGFR2, STAT5 and RUNX2 in breast cancer samples. Materials and Methods: Seventy formalin-fixed and paraffin-embedded breast cancer specimens were processed for immunohistochemistry to detect FGFR2, STAT5 and RUNX2. Staining was scored according to their intensity and their sub cellular localization Results: A strong correlation was observed between nuclear FGFR2 and nuclear STAT5 expression and between nuclear FGFR2 and nuclear RUNX2 expression (p