IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genetic influence on CRISP1 knockout mice phenotype
Autor/es:
WEIGEL MUÑOZ M; BATTISTONE MA; VASEN G; ERNESTO JI; DA ROS V; MALDERA JA; COHEN D; CUASNICU PS
Lugar:
Texas
Reunión:
Congreso; 37th Annual Meeting of American Society of Andrology; 2012
Resumen:
Epididymal protein CRISP1 (Cysteine-RIch Secretory Protein CRISP 1) was identified by our group and proposed to participate in the fertilization process. CRISP1 KO mice generated in our laboratory are fertile although their sperm exhibited lower levels of cAMP, and tyrosine phosphorylation and a reduced ability to interact with the zona pellucida and fuse with the oolema. Considering that different phenotypes can arise from the same mutation depending on the genetic background, in the present work we have investigated the phenotype of CRISP1 KO animals that exhibit a high degree of gene homogeneity. Mice with a mixed C57BL/6-129/SvEv background were subjected to backcrossing with C57BL/6 mice (7 cycles over two years) obtaining animals with a 99.2 % C57BL/6 background. Evaluation of fertility by natural mating revealed a phenotype different from the original hybrid colony as judged as the fact that the time of delivery was significantly greater for females mated with CRISP1−/−males than with CRISP1 KO males. Also differently from the original colony, both fresh and capacitated  CRISP1 KO sperm exhibited significantly lower levels of motility which was restored by exposure of mutant sperm to a cAMP analog (db-cAMP) and a phosphodiesterase inhibitor (3-isobutyl-1-methylxanthine, IBMX). Finally, our studies revealed a significant reduction in the percentage of progesterone-induced acrosome reaction in CRISP1 KO spermatozoa compared to controls which was neither observed in the original colony. Taken together, these results revealed the relevance of CRISP1 for male fertility as well as novel roles in both sperm motility and the acrosome reaction, indicating that CRISP1 KO animals constitute a new example of the influence of the genetic background on the knockout animal phenotype.