NOTCH SYSTEM INVOLVEMENT IN STEROIDOGENESIS, VIABILITY AND MIGRATION OF HUMAN OVARIAN TUMORAL GRANULOSA CELLS (KGN)

PAZOS CAMILA; SCOTTI LEOPOLDINA; ACCIALINI PAULA; TESONE MARTA; IRUSTA GRISELDA

Ciudad de San Juan

Congreso; Segunda Reunión Conjunta de las Sociedades de Biología; 2011

Sociedades de Biología de la República Argentina

Abstract Ovarian cancer is a solid tumor that has a temporally response to treatments. The Notch system is involved in tumoral angiogenesis and it also participates in the survival of tumoral cells. Particularly, Notch 3 and Jagged 2 (members of the Notch system) are increased in ovarian carcinomas. However, there are no studies about the rol of this system in tumoral granulosa cells (TGC). Objectives: To study the function of Notch system in steroidogenesis, apoptosis and migration of human ovarian tumoral granulosa cells (KGN: cell line established from an ovarian granulosa cell carcinoma) and some intracellular pathways. Results: The inhibition of Notch system decreased the synthesis of progesterone and estradiol (p<0,05; p<0,0001, respectively). We also observed an increase in PARP cleavage levels (p<0,05), a decrease in cellular migration (p<0,05), and a decrease in the levels of phospho-AKT (p<0,05). Conclusion: The Notch system is involved in fundamental cellular parameters of TGC, in part trhough activation of PI3K/AKT pathway. We conclude that the Notch system is implicated in the processes studied in human ovarian tumoral granulosa cells.