IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Neonatal treatment with Bisphenol A alters the content of E2, P4 and GnRH-I in ovaries of adult rats
Autor/es:
BOURGUIGNON N.S; BONAVENTURA MM; LUX LANTOS V; LIBERTUN C
Lugar:
San Juan
Reunión:
Congreso; XIII Jornadas Anuales de la Sociedad Argentina de Biología; 2011
Resumen:
BPA is a xenoestrogen used in the manufacture of polycarbonate plastics, epoxy resins and polystyrene. We have previously shown that animals exposed neonatally to BPA have altered serum levels of sex hormones in adulthood: increase E2 and T and decreased P4. In this study, Sprague-Dawley female rats were injected daily sc from the day of birth to 10 postnatal with BPA [500ìg/50ìl (B500) or 50ìg/50ìl (B50) in castor oil], or oil as a control (C), and sacrificed in the morning of estrus at 110-120 days of age. We evaluated the ovarian content of E2, P4, T and GnRH-I by RIA. We found higher ovarian E2 content (pg/mg ovary: C: 2.11±0.55, B50: 12.00±4.28 (p<0.005), B500: 29.76±7.85 (p<0.0005), n= 8-14) and lower P4 (pg/mg ovary: C: 531.28±29.57, B50: 351.59±54.81 (p<0.05), B500: 263.44±56.84 (p<0.005), n= 7-12). T content was not significantly different among groups (pg/mg ovary: C: 17.54±4.57, B50: 11.13±3.09, B500: 8.47±1.72, n= 6-13). In B500, GnRH-I content was increased (pg/mg ovary C: 0.54±0.05, B50: 0.69±0.07, B500: 0.97±0.07; B500: p<0.0005 vs. C, p<0.05 vs. B50, n= 9-11). We conclude that in adult neonatally-BPA treated rats ovarian E2 content, increased, and P4 content, decreased, are altered coinciding with the serum levels previously reported. Ovarian T content did not change, unlike serum levels, suggesting changes in metabolism and/or origin of this steroid. In addition, BPA-treatment increased the ovarian GnRH-I content. These results suggest that neonatal exposure to BPA produces a clear alteration in ovarian GnRH-I and steroidogenesis in adulthood